English
聯系我們
網站地圖
郵箱
舊版回顧



科學家繪制造血干細胞擴增組織的3D轉錄組圖譜

文章來源:動物研究所   發布時間:2019-05-10  【字號:     】  

  血液系統中貯藏著一種具有自我更新、分化成各種血細胞潛能的成體干細胞,稱為造血干細胞,它能夠維持機體長久造血和組織穩態。造血干細胞移植是惡性血癌的有效治療手段,但干細胞來源不足成為限制該治療廣泛應用的瓶頸。因此,造血干細胞的發育,尤其是造血干細胞擴增的研究備受關注。然而,現在的研究主要集中在特定細胞群體或關鍵因子的“線性化”或“平面化”解析,缺乏全面“立體化”的分析。從系統生物學角度解析擴增性造血器官,將為造血干細胞擴增的機制研究提供更多的啟示。鑒于造血發育過程的高度保守性,中國科學院動物研究所劉峰研究組及合作者以斑馬魚尾部造血組織(在功能上類似于哺乳動物的胎肝,為造血干細胞擴增及分化提供了特定的微環境)為研究對象,利用多重轉錄組測序的方法,繪制了擴增性造血器官的3D轉錄組圖譜,闡釋了造血干細胞和微環境細胞之間的分子調控網絡。

  首先,為了系統性地研究斑馬魚尾部造血組織如何支持造血干細胞擴增、分化的機制,劉峰實驗室利用三種轉錄組測序方法,特定類型群體細胞轉錄組、空間轉錄組和單細胞轉錄組,詳細解析了斑馬魚尾部造血組織的時空特性。不同發育時期特定細胞群體的轉錄組測序分析表明,造血干細胞與相應微環境細胞具有協同發育和共享基因表達的特點。空間轉錄組測序反映細胞位置信息,揭示了多種微環境因素對造血干細胞的不同調控方式,并實現了三維轉錄組重構斑馬魚造血器官。單細胞轉錄組數據更精細闡釋尾部造血組織細胞類群、細胞特征及細胞間分子調控,并發現造血干祖細胞分化和細胞周期存在一定的關聯性。多重轉錄組測序綜合分析揭示了造血干細胞在尾部造血組織的擴增受到內源性和外源性信號的共同調控,鑒定了新的造血干細胞的標記基因,發現了影響造血干細胞擴增的信號通路,完善了造血干細胞-微環境細胞間分子調控網絡。例如,染色質結構維持蛋白smchd1,通過調節造血干細胞增殖促進其擴增;微環境細胞來源的Integrin 信號通路負向調控造血干細胞擴增。同時此項工作還構建了斑馬魚尾部造血組織多重轉錄組數據庫(http://www.picb.ac.cn/hanlab/ichtatlas/Home),可以檢索任何基因在造血組織的表達譜,包括基因表達的時間、細胞類型及相對空間位置。

  該工作不僅發現了造血干細胞特征基因、細胞狀態與細胞分化的關系,闡釋了微環境和造血干細胞互作的調控網絡,更為血液系統研究提供了豐富的數據資源;同時,也將為造血干細胞的體外擴增和造血類器官的體外組裝提供理論指導。該研究論文“A 3D atlas of hematopoietic stem and progenitor cell expansion by multi-dimensional RNA-seq analysis430日在線發表于Cell Reports

  動物所博士研究生薛媛媛和中科院計算生物學研究所博士研究生劉登輝為文章共同第一作者,動物所研究員劉峰和計算生物學所研究員韓敬東為文章共同通訊作者。中科院生物化學與細胞生物學研究所研究員景乃禾與中科院廣州生物醫藥與健康研究院研究員彭廣敦在空間轉錄組測序方面提供了大力支持。該研究得到國家自然科學基金委、國家重點基礎研究發展計劃和中科院器官重建與制造戰略性先導科技專項的資助。

 

  圖1:多重轉錄組測序解析斑馬魚造血器官。多重轉錄組測序,包括:特定類型群體細胞轉錄組測序、空間轉錄組測序和單細胞轉錄組測序,揭示斑馬魚尾部造血組織的時空特性。

 

圖2:斑馬魚造血器官轉錄組數據庫 




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

百姓彩票www.bxcp3.com www.888197.com | www.cai3844.com | www.x59.club | www.195661.com | www.886086.com | www.802145.com | www.5871.wang | www.371499.com | www.967674.com | www.990202.com | www.479704.com | www.598023.com | 418466.com | www.gw95.com | www.6429.org | www.75174.com | www.931185.com | www.26rk.com | www.40uv.com | www.3710.net | www.2797.shop | www.982591.com | www.934766.com | www.970894.com | 48eq.com | www.939225.com | www.4411.tv | www.253296.com | www.773162.com | www.741883.com | www.52gj.cc | www.7246.vip | www.1275.vip | www.52527.cc | www.764797.com | 140079.com | www.fm2.cc | www.ve53.com | www.472325.com | www.411899.com | www.714178.com | www.830873.com | www.33739.cc | www.006968.com | www.430889.com | 423618.com | www.7711.ren | www.143594.com | www.14rt.com | www.39631.cc | www.109620.com | www.265344.com | www.637908.com | 779974.com | www.218213.com | www.182702.com | www.981116.com | www.905821.com | www.683552.com | www.761057.com | www.687182.com | www.666750.com | www.2578.cn | www.53157.cc | www.110127.com | www.283611.com | 829249.com | 848776.com | 凤凰彩票www.55228d.com | www.595234.com | 903833.com | 网易彩票www.36788m.com | www.80mm.com | www.9479.vip | www.54041.com | www.296073.com | www.322311.com | www.586985.com | www.431197.com | www.758983.com | 824173.com | 657145.com | 快乐彩票www.35231.com | www.07107.com | www.013215.com | www.262698.com | www.905503.com | 140559.com | 594162.com | www.e41.loan | 大赢家彩票平台www.278965.com | www.5423.cn | 466679.com | www.229630.com | www.293131.com | www.647522.com | www.899844.com | 4185.biz | www.593963.com | www.767325.com | www.909099.cc | www.002283.com | www.030882.com | www.273660.com | 880448.com | www.me20.com | www.12190.com | www.133756.com | www.540616.com | www.605252.com | www.105722.com | www.229912.com | www.390057.com | www.030630.com | www.799981.com | 633075.com | www.jy95.com | www.gp63.com | www.048.biz | www.86793.cc | www.338327.com | www.342323.com | www.7256.biz | 613055.com | www.374132.com | www.96194.com | 127052.com | www.499578.com | www.846270.com | www.864117.com | www.907105.com | www.31975.com | www.237751.com | www.692008.com | www.781020.com | www.035533.com | www.618692.com | 983707.com | www.992259.com | www.879975.com | www.kw45.com | www.7010.cc | www.52001.com | www.581882.com | www.427930.com | www.183833.com | www.590209.com | www.967711.com | www.16524.cc | www.921069.com | www.ji79.com | www.311929.com | www.zs47.com | www.791145.com | www.26vv.com | www.99911.com | www.354174.com | www.513175.com | www.784685.com | www.22754.com | www.504658.com | www.736362.com | 124030.com | 232712.com | 25144.com | 251830.com | www.76fj.com | www.54iu.com | www.46649.com | www.339831.com | www.454100.com | www.0676.org | www.36987.com | www.238191.com | www.362992.com | 028132.com | www.693365.com | www.831757.com | www.891229.com | www.688816.com | www.835231.com | www.4812.cc | www.491178.com | www.557796.com | www.018576.com | 948752.com | 大赢家彩票平台www.810926.com | www.54422.com | www.464077.com | 001835.com | 515560.com | www.hlfvip91.com | 897472.com | 43bl.com | www.577739.com | 683591.com | www.ng97.com | www.098873.com | www.065527.com | www.612569.com | www.983954.com | 654455.com | www.92pg.com | www.9179.com | www.240340.com | www.877832.com | www.658278.com | www.4095.biz | www.00qw.com | www.32597.cc | www.972729.com | www.813255.cc | www.37291.com | www.267315.com | www.791885.com | 571165.com | 924700.com | www.907258.com | www.811974.com | www.742888.com | 173203.com | 993984.com | cai9222.com | www.79878.com | 061091.com | www.nm79.com | www.399228.cc | 984223.com | www.19rc.com | 博乐彩票www.35918m.com | www.75qg.com | www.1130.vip | www.1151.me | www.8106.tv | www.62gd.com | www.bt40.com | 617704.com | 340494.com | www.865732.com | cai1112.com | 1253.com | 024712.com | www.349483.com | www.092726.com | www.731026.com | 935779.com | www.884023.com | rn05.com | 786553.com | www.052285.com | www.95523.cc | 579464.com | www.065891.com | www.nb45.com | www.60628.com | www.493528.com | www.iu90.com | www.l73.cc | www.c55.wang | www.335.red | www.991521.com | www.65or.com | www.8822.cc | www.0022.cc | www.005522.cc | www.117447.com | www.zh2.cc | www.587288.com | www.653109.com | www.712136.com | www.882606.com | www.lz56.com | www.82568.cc | 954298.com | www.067585.com | www.12hk.com | www.736.online | www.50618.com | 731449.com | www.433199.com | www.091338.com | 272049.com | www.06575.com | www.825635.com | lj38.com | www.zx85.com | www.012.me | www.30sg.com | www.928087.com | www.694577.com | www.141423.com | www.402.website | www.70094.com | www.msc88.com | 306960.com | 781643.com | www.ql44.com | www.350380.com | www.2520.vip | www.772494.com | www.4255.vip | www.414244.com | www.37444.com | www.0162.com | www.3792.biz | www.028351.com | www.162949.com | www.550813.com | www.906186.com | www.91uw.com | www.42599.com | www.272533.com | www.d68.top | www.61293.cc | www.0fu.com | www.949907.com | www.35tk.cc | www.35kh.com | 444494.com | www.479552.com | www.7782.cc | www.789672.com | www.753356.com | www.27125.com | www.ug10.com | www.378885.com | 18484.com | 78774.com | 452251.com | 596356.com | 982418.com | www.r32.com | www.dg91.com | www.234244.com | 37111.com | 206159.com | 16758.com | 696190.com | 500彩票www.347177.com | www.y60.net | www.408804.com | 434384.com | www.37ul.com | www.760779.com | www.900202.com | www.920213.com | www.29586.com | www.34562.com | 448494.com | www.885076.com | www.553676.com | www.254281.com | www.52787.cc | www.829364.com | 818合彩www.www.5666hc.com | www.598535.com | www.632358.com | www.331721.com | www.uy09.com | 853746.com | www.as02.com | www.86089.com | www.189000.com | 859507.com | 958460.com | www.444530.com | www.0788.me | www.064022.com | 813093.com | www.52738.com | 49678.com | 18luck.tv | www.c2.tv | www.581335.com | www.548776.com | www.252318.com | 740668.com | 362335.com | 113430.com | 58820.com | 791531.com | 720565.com | 818725.com | 576924.com | www.n34.biz | 266075.com | www.215148.com | 669929.com | www.80kj.cc | www.yn19.com | www.8920.online | www.82lz.com | www.288657.com | www.78844.com | www.520538.com | www.434578.com | 408872.com | www.hv128.com | 114654.com | 582859.com | 894965.com | www.54ry.com | www.460.in | www.111116.com | www.379535.com | www.749520.com | www.979388.com | www.685075.com | www.593522.com | 099954.com | www.953365.com | www.57861.cc | www.486898.com | www.98090.com | www.074996.com | 730730.cc | 890521.com | 484805.com | 风之彩www.88fzc.com | www.126788.com | www.109678.com | www.365698.cc | www.950217.com | www.590896.com | 654364.com | www.541141.com | www.469557.com | 431408.com--15876.com | 鸿利彩票www.588088a.com | 645299.com | www.444726.com | www.808801.com | www.62026.com | www.203368.com | www.229316.com | www.282662.com | www.670640.com | 34339.com | www.883131.com | www.96922.com | www.944182.com | www.617637.com | www.395496.com | www.795536.com | www.182048.com | 137689.com | 543811.com | 88914.com | www.17.com | www.37815.cc | www.660306.com | www.154979.com | 488633.com | 5052.biz | 026495.com | 413171.com | www.30od.com | www.29988.com | www.83370.com | www.87791.cc | www.634476.com | 好彩www.60123s.com | www.31219.com | www.xq05.com | www.89yf.com | www.22539.com | www.iv9.com | www.008145.com | www.1983.org | www.796597.com | www.kw22.com | www.yn66.com | www.514120.com | www.ra28.com | www.370252.com | www.416505.com | www.288715.com | www.246288.com | 488308.com | www.994721.com | www.817672.com | www.gd23.com | www.ut81.com | www.044629.com | www.167864.com | www.599680.com | www.981250.com | www.822571.com | pv75.com | 555499.com | www.973263.com | www.812080.com | www.127147.com | www.583.date | www.71wh.com | www.012908.com | 144676.com | www.949835.com | www.675146.com | www.456231.com | www.84pj.cc | 金冠彩票www.c4525.com | www.sb5208.com | www.97533.cc | www.55819.com | www.096488.com | www.082968.com | www.620303.com | 500彩票www.500231.com | 871649.com | www.ie73.com | www.10re.com | www.9085.org | www.48091.com | www.18kt.com | www.137692.com | www.179563.com | www.99345.com | www.176689.com | www.9190.loan | www.1857.wang | www.582775.com | www.442269.com | www.654424.com | www.458040.com | 凤凰彩票www.66652f.com | www.cp311.cc | www.bn92.com | www.3wx.cc | www.52185.cc | www.319218.com | www.201153.com | www.023502.com | www.20552.com | www.019670.com | 25401.com | www.07ey.com | www.df09.com | www.68354.com | www.49940.com | www.114274.com | www.082688.com | www.22635.com | www.2120.vip | www.0370.loan | www.1xu.cc | www.717847.com | www.3276.cn | www.496333.cc | www.381112.com | www.197187.com | 4050.cc | www.017184.com | www.093549.com | www.69711.cc | www.251502.com | www.171302.com | www.133124.com | www.07651.com | www.871220.com | www.587890.com | www.606689.com | www.8052.vip | www.39359.cc | www.2160.vip | www.766.in | www.3oy.com | www.250157.com | www.460476.com | www.588195.com | www.2335.biz | www.88078.cc | www.722111.com | www.585760.com | www.958689.com | www.649558.com | www.291921.com | www.081821.com | www.69440.com | www.057208.com | www.255962.com | www.482344.com | 535384.com | 141083.com | www.6112.cm | www.999.org | www.493785.com | www.396415.com | 036163.com | 吉利彩票www.66376n.com | www.9tw.com | www.gu51.com | www.ut8.com | 961841.com | 954796.com | www.955925.com | www.735747.com | www.699736.com | www.667113.com | www.671110.com | www.783282.com | www.700197.com | www.800463.com | www.736821.com | www.672579.com | www.67uw.com | 293639.com | 271639.com | 744847.com | 688彩票www.688dl.com | zb38.cc | 456544.com | www.951306.com | 227874.com | 130392.com | www.v80.top | www.94197.com | www.262901.com | www.496900.com | www.307210.com | www.203434.com | www.123237.com | www.440674.com | www.493135.com | www.365832.cc | www.423855.com | www.295704.com | www.595795.com | www.939759.com | www.970295.com | www.719840.com | www.989334.com | www.6988.win | www.5404.org | www.247899.com | www.348556.com | 516666.com | 119720.com | www.907053.com | www.679708.com | www.542802.cc | www.658625.com | www.524355.com | www.254936.com | www.127064.com | www.32180.com | www.703891.com | www.198034.com | 829999.com | 230866.com | www.981148.com | 127141.com | www.926844.com | www.703996.com | www.423178.com | www.868477.com | 225108.com | www.977001.com | www.898393.com | www.679572.com | www.hy97.com | 盛彩网www.41518w.com | www.hy06.com | www.006548.com | 9148.com | www.103104.com | www.62628.com | www.5575.cc | www.77vg.com | www.ju05.com | www.4654.cm | www.346438.com | www.uj92.com | www.568203.com | www.966202.com | 365681.cc | www.cp9757.com | 920061.com | www.jn.com | 536769.com | www.008.red | www.99pz.com | www.015035.com | www.8231.top | www.98zm.com | www.ne18.com | 彩多多www.78949i.com | www.35ki.com | www.2535.xyz | www.1nk.com | www.k80.shop | www.600487.com | www.8569.top | www.883890.com | www.7639.loan | www.71414.com | www.24701.com | www.1036.cm | www.23122.com | www.wx96.com | 892321.com | www.850766.com | www.661783.com | www.566075.com | 623670.com | 889616.com | www.093018.com | www.379850.com | www.562253.com | 613966.com | www.613.live | www.8915.xyz | www.944.vip | www.044559.com | www.974049.com | 679752.com | www.828230.com | www.566480.com | www.369540.com | 745172.com | 08450.com | www.017025.com | 851192.com | www.501819.com | www.021730.com | 9386.biz | 854163.com | 234309.com | www.zl.com | www.k97.com | www.199995.com | www.103310.com | www.078087.com | www.743249.com | www.589915.com | www.217086.com | www.120715.com | www.023569.com | www.007386.com | www.995128.com | 57414.com | 320707.com | www.941030.com | www.652921.com | 973143.com | www.60xw.com | www.cs61.cc | www.6633.cn | www.808560.com | www.590680.com | www.ji92.com | www.3726.net | www.86ur.com | www.4463.vip | www.9561.vip | www.349.cm | www.12ru.com | www.wr61.com | 043571.com | 183016.com | 567733.com | www.1355.bid | 751442.com | www.650658.com | www.85239.com | www.810198.com | 655251.com | www.805781.com | 81022.com | www.569235.com | www.80215.com | www.i44.biz | www.526311.com | www.208578.com | www.960714.com | www.fm08.com | www.239000.com | 99662.com | 650326.com | 278085.com | 810433.com | 954901.com | 26278.com | 447326.com | 075351.com | 130381.com | www.815885.com | 085230.com | www.709110.com | www.833828.com | 332507.com | www.ms33.com | 975430.com | www.340406.com | www.0939.wang | www.jv21.com | www.21712.com | www.52916.cc | www.06001.com | www.525097.com | www.750048.com | www.769268.com | www.927607.com | www.666630.com | www.618665.com | www.71fo.com | www.6019.in | www.x19.bid | www.eu85.com | www.ge.com | 654675.com | 294152.com | www.718553.com | www.673506.com | 560902.com | www.957614.com | 657834.com | 685706.com | 411191.com | www.680598.com | www.250528.com | www.20740.com | www.vn55.com | 086689.com | 83150.cc | www.654765.com | www.655772.com | www.205234.com | www.527237.com | 860823.com | www.gt13.com | www.294.me | www.nl37.com | www.st65.com | 728885.com | 945545.com | www.8738.biz | www.549319.com | www.hv64.com | 096522.com | 013037.com | www.819656.com | www.390082.com | www.67gf.com | www.679191.com | www.374198.com | www.445408.com | www.279269.com | www.666997.com | www.261214.com | www.7035.org | www.iz42.com | 631897.com | www.86im.com | www.275078.com | www.127569.com | www.1146.vip | 783759.com | 鸿运彩票www.hy5501.com | 706836.com | www.862220.com | 909369.com | 779455.com | www.xq13.com | www.84hw.com | www.73508.com | www.82858.com | www.uh60.com | www.377186.com | www.47rq.com | 717860.com | 297097.com | www.94381.com | www.56om.com | www.69576.cc | www.1489.vip | www.92vv.cc | 汇丰彩票www.hf0688.com | www.41wn.com | www.002674.cc | 352208.cc | www.616734.com | www.9610.com | www.980153.com | www.99488.com | 662477.com | www.3769.vip | www.779796.com | www.03ve.com | www.196892.com | www.057885.com | www.556999.com | 766456.com | www.53182.cc | www.446402.com | www.181859.com | www.511011.com | www.006784.com | www.98938.com | www.040025.com | 网易彩票www.36788x.com | www.018465.com | www.5285.shop | 500万彩票www.www.98528f.com | 706323.com | 830320.com | www.721213.com | www.762242.com | www.347200.com | www.190082.com | www.86351.cc | www.112031.com | www.402088.com | www.507557.com | www.333952.com | www.285867.com | www.276161.com | www.106207.com | www.4379.com | www.087357.com | www.4649.xyz | www.rh54.com | 951864.com | www.811413.com | www.7005.vip | www.8hw.cc | www.207922.com | www.095795.com | www.33707.com | www.523785.com | www.900700.com | www.659114.com | www.785152.com | www.248177.com | www.9366.vip | 幸运彩票www.716726.com | www.122836.com | www.48jd.com | www.60416.com | www.70204.com | www.29728.com | www.7393.org | www.775119.com | www.470373.com | www.467268.com | www.300111.com | www.596989.com | www.671493.com | www.72818.com | 496201.com | www.730578.com | www.337355.com | www.885043.com | www.679585.com | www.544758.com | www.14rn.com | www.f33.top | www.6517.xyz | www.275266.com | www.6567.pw | 04974.cc | www.982343.com | www.381580.com | www.037871.com | www.977070.com | www.716753.com | 彩民村www.77cmc.com | www.310030.com | www.819581.com | www.541622.com | www.068607.com | www.366922.com | www.199001.com | www.371807.com | www.538368.com | www.464949.com | www.344987.com | www.092068.com | www.972184.com | www.701063.com | www.323779.com | www.072398.com | www.8775.loan | www.2578.biz | www.82790.com | www.009272.com | www.181005.com | www.7858.biz | www.cp194.cc | www.971497.com | 758983.com | www.898793.com | www.576573.com | www.200081.cc | www.107083.com | www.985685.com | www.002794.com | 948207.com | www.123470.com | www.196111.com | www.76057.com | www.26216.cc | www.436738.com | www.312054.com | www.32896.cc | www.32aq.com | 971583.com | www.207297.com | www.cp162.com | www.909728.com | www.vu22.com | www.441130.com | www.29ea.com | www.217072.com | www.141919.com | www.576344.com | www.445991.com | www.185198.com | www.43430.cc | www.102310.com | www.49045.com | www.31217.cc | www.6737.pro | www.253625.com | www.005628.com | www.41317.com | www.59089.com | www.566680.com | www.15451.com | www.224241.com | www.92178.cc | 739499.com | www.329922.com | 802622.com | www.261443.com | www.996945.com | 028425.com | www.544059.com | www.70673.com | www.ut65.com | 9001.ent | 226798.com | 福利彩票www.flff9.com | www.861120.com | www.921445.com | www.508319.com | www.110615.com | www.9970.org | 523836.com | www.82xv.com | 608885.com | www.763969.com | www.501040.com | www.88312.com | 465684.com | www.089711.com | 251371.com | www.259215.com | 682087.com | www.940908.com | www.298385.com | www.17323.cc | 615471.com | www.334808.com | www.189469.com | www.805383.com | 588044.com | 646141.com | 657943.com | www.373971.com | www.8275.xyz | www.802084.com | www.69085.com | www.977519.com | www.111202.com | www.36536.cc | www.yn95.com | www.184744.com | www.970161.com | www.645778.com | 095519.com | www.156365.cc | www.39555.com | www.13lk.com | www.680587.com | 215174.com | 397160.com | www.948807.com | www.719371.com | www.601614.com | www.911938.com | www.584902.com | www.221484.com | 325844.com | www.486561.com | www.kh83.com | 022354.com | www.501953.com | 654241.com | www.68bi.com | 500万彩票www.39500u.com | 717210.com | www.980774.com | 幸运彩票www.672766.com | 739272.com | www.853428.com | 788740.com | www.961337.com | www.yb04.com | 937921.com | 318189.com | www.639168.com | www.316288.com | www.4825.vip | www.cp2205.com | www.652973.com | www.705977.com | www.127379.com | www.145225.com | www.302302.cc | www.44sq.com | 6529.biz | www.564813.com | www.2869.vip | www.192014.com | www.232799.com | www.013244.com | www.28781.cc | www.01uy.com | www.612981.com | www.203655.com | www.560527.com | www.984683.com | www.696317.com | www.483909.com | www.184351.com | www.116225.com | 697903.com | 274395.com | www.668993.com | www.637557.com | www.12sb.com | 655251.com | www.948325.com | www.451605.com | www.387755.com | www.417803.com | www.006464.com | www.523597.com | www.200805.com | www.6703.cn | 澳客网www.okw8.com | 538692.com | 438811.com | 109249.com | www.95nk.com |